In vitro propagation of Annona deceptrix (westra) H. Rainer (Annonaceae), an endangered species in Ecuador

Authors

  • María Auxiliadora Nevárez Loor , Instituto de Posgrado, Universidad Técnica de Manabí, Portoviejo, Ecuador
  • Fátima Consuelo Macías Ponce Facultad de Ingeniería Agronómica, Universidad Técnica de Manabí. Km. 13.5 vía Portoviejo-Santa Ana. Manabí, Ecuador https://orcid.org/0000-0001-5090-5252
  • José Pico-Mendoza Facultad de Ingeniería Agronómica, Universidad Técnica de Manabí. Km. 13.5 vía Portoviejo-Santa Ana. Manabí, Ecuador https://orcid.org/0000-0002-3276-1678

DOI:

https://doi.org/10.70099/BJ/2024.01.01.17

Keywords:

Annona deceptrix, propagation, in vitro rooting, ex-vitro acclimatization, growth regulators

Abstract

This work aims to develop a protocol for the in vitro propagation of Annona deceptrix  (westra) H. Rainer. In addition, it measures survival in ex-vitro conditions. Nodal shoot segments were used as explants, disinfected with different NaClO concentrations and exposure times, and then precultured in the Murashige and Skoog (MS) basal culture medium. Woody Plant Medium (WPM) was used for the multiplication and root-ing phase, with different types and concentrations of phytoregulators. In the establishment phase, the ex-plants responded better with the 1% NaClO treatment for 15 minutes, where the highest survival percentage was observed at 72.04%. For the variable number of shoots and stem diameter, the treatment with BAP (1 mgL-1) + (GA3 0.25 gL-1) + (WPM) reached the best average with a value of 2.00 and 2.35, respectively. Mm, respectively. For root induction, the best response was obtained when 3mgL-1 IBA (T3) was added to the culture medium, reaching an average root length of 5.75 cm after 60 days of culture in the medium. The rooted Vitro plants of an approximate height of 5 cm were brought to the mesh house for their acclimatization; in this phase, the survival rate was evaluated during the first week of acclimatization, and the plant's growth fifteen days after sowing in trays containing a peat-based substrate, where T3 had an average survival of 70% and higher plant height at 60 days, indicating that this treatment promoted faster seedling development under conditions ex-vitro.

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Published

2024-02-15

How to Cite

Nevárez Loor , M. A., Macías Ponce, F. C., & Pico-Mendoza, J. (2024). In vitro propagation of Annona deceptrix (westra) H. Rainer (Annonaceae), an endangered species in Ecuador. BioNatura Journal: Ibero-American Journal of Biotechnology and Life Sciences, 1(1), 12. https://doi.org/10.70099/BJ/2024.01.01.17

Issue

Vol. 1 No. 1 (2024)

Section

Research Articles

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