Evaluation of Arbuscular Mycorrhizal Fungi Associated with Miconia bracteolata Bonpl case study High Andean Forest, Ecuador

Authors

  • Diego Armando Damián-Carrión Escuela Superior Politécnica de Chimborazo, Grupo de Investigación y Desarrollo para el Ambiente y Cambio Climático (GIDAC). Panamericana Sur km 1 1/2, Riobamba-Ecuador; Programa de Doctorado Internacional en Agricultura y Medioambiente para el Desarrollo, Universidad Santiago de Compostela, Escuela Politécnica Superior, Campus Universitario S/n, 27002-Lugo, España https://orcid.org/0000-0002-1545-7439
  • Carla Fernanda Silva-Padilla Facultad de Ingeniería/Ingeniería Ambiental, Universidad Nacional de Chimborazo, Ecuador-UNACH, Riobamba, Ecuador https://orcid.org/0000-0002-2105-7263
  • Franklin Enrique Cargua-Catagña Programa de Doctorado Internacional en Agricultura y Medioambiente para el Desarrollo, Universidad Santiago de Compostela, Escuela Politécnica Superior, Campus Universitario S/n, 27002-Lugo, España.
  • Jorge Reyes Reyes Cuerpo Académico en Recursos Forestales, Facultad de Ciencias Agrícolas, Universidad Autónoma de Chiapas. Entronque Carretera costera y Estación Huehuetán; Huehuetán, Chiapas, México. C.P. 30670
  • Fernando Paredes-Arcos Programa de Estadística Aplicada, Universidad Politécnica Estatal del Carchi. Carchi-Ecuador https://orcid.org/0000-0001-5532-7850

DOI:

https://doi.org/10.70099/BJ/2024.01.03.10

Keywords:

Ecuadorian Andes, Andean brow forests, floristic diversity indices, mycorrhizal fungal morphotypes

Abstract

The research aims to identify the arbuscular mycorrhizal fungi (AMF) associated with Miconia bracteolata Bonpl and determine the ecological parameters. The analysis focuses on the Guangra sector, located in the buffer zone of the Sangay National Park - Ecuador, using the most modern methods for the extraction and quantification of AMF. The highest spore populations were found in plot 03 (5637.95 spores/100 g soil), while the lowest was found in plot 01 (3586.46 spores/100 g soil), with a mean colonization percentage of 15.69, developed in a soil type with a slightly acidic pH, which is strongly associated with AMF spore survival. In plot 03, the genus Glomus spp. showed high importance for ecological parameters, medium diversity H-index, and high dominance Simpson's diversity index. These results can help to predict distributions and the degree of ecosystem disturbance, ultimately contributing to the conservation of biodiversity and functions in high Andean ecosystems.

References

1. Luna-Florin AD, Nole-Nole DA, Rodríguez-Caballero E, et al. Ecological characterization of the flora in Reserva Ecológica Arenillas, Ecuador. Applied Sciences 2022; 12(17):8656; doi: 10.3390/app12178656.

2. Zuluaga S, Vargas FH, Aráoz R, et al. Main aerial top predator of the andean montane forest copes with fragmentation, but may be paying a high cost. Glob Ecol Conserv 2022; 37:02174; doi:10.1016/j.gecco.2022.e02174.

3. Goldenberg R, Penneys DS, Almeda F, et al. Phylogeny of Miconia (Melastomataceae): Patterns of stamen diversification in a megadiverse neotropical genus. Int J Plant Sci 2008;169(7):963–979; doi: 10.1086/589697.

4. Camenzind T, Homeier J, Dietrich K, et al. Opposing effects of nitrogen versus phosphorus additions on my-corrhizal fungal abundance along an elevational gradient in tropical montane forests. Soil Biol Biochem 2016; 94:37– 47; doi: 10.1016/j.soilbio.2015.11.011.

5. Stefanowicz AM, Kapusta P, Stanek M, et al. Herbaceous plant species and their combinations positively affect soil microorganisms and processes and modify soil physicochemical properties in a mesocosm experi-ment. For Ecol Manage 2023; 532:120826; doi: 10.1016/j.foreco.2023.120826.

6. Huang B, Zhang L, Cao Y, et al. Effects of land-use type on soil organic carbon and carbon pool management index through arbuscular mycorrhizal fungi pathways. Glob Ecol Conserv 2023; 43:02432; doi: 10.1016/j.gecco.2023.e02432.

7. Carron AI, Garibaldi LA, Marquez S, et al. The soil fungal community of native woodland in Andean Pata-gonian forest: A case study considering experimental forest management and seasonal effects. For Ecol Manage 2020; 461:117955; doi: 10.1016/J.FORECO.2020.117955.

8. Dey M, Ghosh S. Arbuscular mycorrhizae in plant immunity and crop pathogen control. Rhizosphere 2022; 22:100524; doi: 10.1016/j.rhisph.2022.100524.

9. Lozano Sánchez JD, Armbrecht I, Montoya Lerma J. Hongos formadores de micorrizas arbusculares y su efecto sobre la estructura de los suelos en fincas con manejos agroecológicos e intensivos. Acta Agron 2015;64(4); doi: 10.15446/acag.v64n4.46045.

10. Leocadio C, Álvarez-López N, Barrios A, et al. Soil culturable microbial diversity in an undisturbed montane cloud forest of Oaxaca, Mexico. Rev Mex Biodivers 2023; 94:944980; doi: 10.22201/ib.20078706e.2023.94.4980.

11. Wang Y, Zhang W, LI C, et al. Long-term nitrogen and/or phosphorus additions lead to a shift in the diversity of soil arbuscular mycorrhizal fungi and improve rainfed crop yield on the Chinese Loess Plateau. Pe-dosphere 2023; doi: 10.1016/j.pedsph.2023.01.009.

12. Fang M, Liang M, Liu X, et al. Abundance of saprotrophic fungi determines decomposition rates of leaf litter from arbuscular mycorrhizal and ectomycorrhizal trees in a subtropical forest. Soil Biol Biochem 2020; 149:107966; doi: 10.1016/j.soilbio.2020.107966.

13. Zubek S, Rożek K, Stefanowicz AM, et al. The impact of beech and riparian forest herbaceous plant species with contrasting traits on arbuscular mycorrhizal fungi abundance and diversity. For Ecol Manage 2021; 492:119245; doi: 10.1016/j.foreco.2021.119245.

14. Yang X, Ma Y, Zhang J, et al. How arbuscular mycorrhizal fungi drives herbaceous plants’ C: N: P stoichiom-etry? A meta-analysis. Science of The Total Environment 2023; 862:160807; doi: 10.1016/j.scitotenv.2022.160807.

15. Segovia-Cruz JA, Souza V, Mercado-Flores Y, et al. Microbial diversity of the Tecozautla geyser, Mexico. Rev Mex Biodivers 2023; 94:945131; doi: 10.22201/ib.20078706e.2023.94.5131.

16. Lee E-H, Eo J-K, Ka K-H, et al. Diversity of Arbuscular Mycorrhizal Fungi and Their Roles in Ecosystems. Mycobiology 2013;41(3):121–125; doi: 10.5941/MYCO.2013.41.3.121.

17. Sharma MP, Buyer JS. Comparison of biochemical and microscopic methods for quantification of arbuscular mycorrhizal fungi in soil and roots. Applied Soil Ecology 2015; 95:86–89; doi: 10.1016/j.apsoil.2015.06.001.

18. García VJ, Márquez CO, Isenhart TM, et al. Evaluating the conservation state of the páramo ecosystem: An object-based image analysis and CART algorithm approach for central Ecuador. Heliyon 2019;5(10); doi: 10.1016/j.heliyon.2019.e02701.

19. Alvis Gordo JF. Análisis estructural de un bosque natural localizado en zona rural del municipio de Popayán. Biotecnología en el sector Agropecuario y Agroindustrial 2009;7(1):115–122.

20. Simpson EH. Measurement of Diversity. Nature 1949 ;163(4148):688–688; doi: 10.1038/163688a0.

21. Daly A, Baetens J, De Baets B. Ecological Diversity: Measuring the Unmeasurable. Mathematics 2018; 6(7):119; doi: 10.3390/math6070119.

22. Chaudhary VB, Holland EP, Charman-Anderson S, et al. What are mycorrhizal traits? Trends. Ecol Evol 2022; 37(7):573–581; doi: 10.1016/J.TREE.2022.04.003.

23. Stover HJ, Naeth MA, Boldt-Burisch K. Soil disturbance changes arbuscular mycorrhizal fungi richness and composition in a fescue grassland in Alberta Canada. Applied Soil Ecology 2018; 131:29–37; doi: 10.1016/j.apsoil.2018.07.008.

24. Moebius-Clune DJ, Moebius-Clune BN, van Es HM, et al. Arbuscular mycorrhizal fungi associated with a single agronomic plant host across the landscape: Community differentiation along a soil textural gradient. Soil Biol Biochem 2013; 64:191–199; doi: 10.1016/j.soilbio.2012.12.014.

25. Nguvo KJ, Gao X. Weapons hidden underneath: bio-control agents and their potentials to activate plant in-duced systemic resistance in controlling crop Fusarium diseases. Journal of Plant Diseases and Protection 2019;126(3):177–190; doi: 10.1007/s41348-019-00222-y.

26. Seiwa K, Negishi Y, Eto Y, et al. Successful seedling establishment of arbuscular mycorrhizal-compared to ectomycorrhizal-associated hardwoods in arbuscular cedar plantations. For Ecol Manage 2020; 468:118155; doi: 10.1016/J.FORECO.2020.118155.

27. Xiao D, Chen Y, He X, et al. Temperature and precipitation significantly influence the interactions between arbuscular mycorrhizal fungi and diazotrophs in karst ecosystems. For Ecol Manage 2021; 497:119464; doi: 10.1016/j.foreco.2021.119464.

28. Liu Z, Bai J, Qin H, et al. Application of rice straw and horse manure coameliorated soil arbuscular mycor-rhizal fungal community: Impacts on structure and diversity in a degraded field in Eastern China. Land Degrad Dev 2021; 32(8):2595–2605; doi: 10.1002/ldr.3927.

29. Qin Z, Zhang H, Feng G, et al. Soil phosphorus availability modifies the relationship between AM fungal diversity and mycorrhizal benefits to maize in an agricultural soil. Soil Biol Biochem 2020; 144:107790; doi: 10.1016/j.soilbio.2020.107790.

30. Moína-Quimí E, Oviedo-Anchundia R, Nieto-Barciona S, et al. Evaluación de los Hongos Micorrízicos Ar-busculares de zonas del trópico húmedo del Ecuador. Bionatura 2018; 3(1); doi: 10.21931/RB/2018.03.01.9.

31. Johnson NC, Wilson GWT, Wilson JA, et al. Mycorrhizal phenotypes and the Law of the Minimum. New Phytologist 2015;205(4):1473–1484; doi: 10.1111/nph.13172.

32. Haug I, Setaro S, Suárez JP. Arbuscular Mycorrhizal Fungi and Ectomycorrhizas in the Andean Cloud Forest of South Ecuador. The Andean Cloud Forest 2021; 111–129; doi: 10.1007/978-3-030-57344-7_6/FIGURES/8.

33. Bainard LD, Klironomos JN, Gordon AM. Arbuscular mycorrhizal fungi in tree-based intercropping systems: A review of their abundance and diversity. Pedobiologia (Jena) 2011; 54(2):57–61; doi: 10.1016/j.pedobi.2010.11.001.

34. Chiquito Noboa I, Naranjo Morán J, Barcos-Arias M. Presencia de hongos micorrízicos arbusculares (HMA) en especies representativas del bosque seco tropical del litoral ecuatoriano. Bionatura 2018;3(1); doi: 10.21931/RB/2018.03.01.7.

35. Chen J, Heikkinen J, Hobbie EA, et al. Strategies of carbon and nitrogen acquisition by saprotrophic and ec-tomycorrhizal fungi in Finnish boreal Picea abies-dominated forests. Fungal Biol 2019; 123(6):456–464; doi: 10.1016/j.funbio.2019.03.005.

36. Caicedo Rosero DM, Benavides Rosales HR, Carvajal Pérez LA, et al. Población de macrofauna en sistemas silvopastoriles dedicados a la producción lechera: análisis preliminar. La Granja 2017; 27(1):77–85; doi: 10.17163/lgr.n27.2018.06.

37. Lazo JA, Valdés NV, Sampaio RA, et al. Diversidad zoológica asociada a un silvopastoreo leucaena-guinea con diferentes edades de establecimiento. Pesqui Agropecu Bras 2007; 42(12):1667–1674; doi: 10.1590/S0100-204X2007001200001.

38. Zhao Z, Wei X, Wang X, et al. Concentration and mineralization of organic carbon in forest soils along a climatic gradient. For Ecol Manage 2019; 432:246–255; doi: 10.1016/J.FORECO.2018.09.026.

39. Estrada B, Beltrán-Hermoso M, Palenzuela J, et al. Diversity of arbuscular mycorrhizal fungi in the rhizo-sphere of Asteriscus maritimus (L.) Less., a representative plant species in arid and saline Mediterranean ecosystems. J Arid Environ 2013; 97:170–175; doi: 10.1016/j.jaridenv.2013.05.019.

40. Longo S, Nouhra E, Goto BT, et al. Effects of fire on arbuscular mycorrhizal fungi in the Mountain Chaco Forest. For Ecol Manage 2014; 315:86–94; doi: 10.1016/j.foreco.2013.12.027.

41. Camenzind T, Rillig MC. Extraradical arbuscular mycorrhizal fungal hyphae in an organic tropical montane forest soil. Soil Biol Biochem 2013; 64:96–102; doi: 10.1016/j.soilbio.2013.04.011.

42. Montero Sommerfeld H, Díaz LM, Alvarez M, et al. High winter diversity of arbuscular mycorrhizal fungal communities in shallow and deep grassland soils. Soil Biol Biochem 2013; 65:236–244; doi: 10.1016/j.soilbio.2013.06.002.

43. Kohout P, Sudová R, Janoušková M, et al. Comparison of commonly used primer sets for evaluating arbus-cular mycorrhizal fungal communities: Is there a universal solution?. Soil Biol Biochem 2014; 68:482–493; doi: 10.1016/J.SOILBIO.2013.08.027.

44. Püschel D, Kolaříková Z, Šmilauer P, et al. Survival and long-term infectivity of arbuscular mycorrhizal fun-gi in peat-based substrates stored under different temperature regimes. Applied Soil Ecology 2019; 140:98–107; doi: 10.1016/J.APSOIL.2019.04.020.

45. Wang J, Wang GG, Zhang B, Yuan Z, Fu Z, Yuan Y, Zhu L, Ma S, Zhang J. Arbuscular Mycorrhizal Fungi Associated with Tree Species in a Planted Forest of Eastern China. Forests. 2019; 10(5):424. https://doi.org/10.3390/f10050424

46. Mahdhi M, Tounekti T, Abada E, Al‐Faifi Z, Khemira H. Diversity of arbuscular mycorrhizal fungi associated with acacia trees in southwestern Saudi Arabia. J Basic Microbiol. 2020 Apr 16;60(4):322–30. https://doi.org/10.1002/jobm.201900471

47. Dalli Y; Yahia N; Bekki A. Diversity of arbuscular mycorrhizal fungi associated with carob trees (Ceratonia Siliqua L.) in Western Algeria. Plant Cell Biotechnology and Molecular Biology. 2020, p. 180-193.

48. Lugo M.A, Ontivero R.E; Iriarte H.J; Yelikbayev, B.; Pagano, M.C. The Diversity of Arbuscular Mycorrhizal Fungi and Their Associations in South America: A Case Study of Argentinean and Brazilian Cattle Raising Productive Ecosystems: A Review. Diversity 2023, 15, 1006. https://doi.org/10.3390/d15091006

49. Jie W, Baiyan C, and Jingping G. Molecular detection and community analysis of arbuscular mycorrhizal fungi in the rhizosphere of Phellodendron amurense. Annals of microbiology. 2012: 1769-1777.

50. Marleau, J., Dalpé, Y., St-Arnaud, M., & Hijri, M. Spore development and nuclear inheritance in arbuscular mycorrhizal fungi. BMC evolutionary biology. 2011. 11, 1-11.

51. Boyno G, Demir S, Rezaee Danesh Y, Durak ED, Çevik R, Farda B, Djebaili R, Pellegrini M. A New Technique for the Extraction of Arbuscular Mycorrhizae Fungal Spores from Rhizosphere. Journal of Fungi. 2023; 9(8):845. https://doi.org/10.3390/jof9080845

52. Erazo N, Manzano J and Patiño B. Caracterización Molecular de la Diversidad Fúngica de los Bosques Llucud y Palictahua: Potencialidades en Control Biológico/Molecular Characterization of Diversity Fungic of the Llucud and Palictahua Forests: Potential in Biological Control. KnE Engineering. 2020. 313-328.

53. Singavarapu B, ul Haq H, Darnstaedt F, Nawaz A, Beugnon R, Cesarz S, et al. Influence of tree mycorrhizal type, tree species identity, and diversity on forest root‐associated mycobiomes. New Phytologist. 2024 May 24;242(4):1691–703. https://doi.org/10.1111/nph.19722

54. Herrmann L, Lesueur D, Bräu L, Davison J, Jairus T, Robain H, et al. Diversity of root-associated arbuscular mycorrhizal fungal communities in a rubber tree plantation chronosequence in Northeast Thailand. Mycorrhiza. 2016 Nov 23;26(8):863–77. https://doi.org/10.1007/s00572-016-0720-5

55. Gao X, Lv J, Guo C, Hu A, Wu X, Li Z. Species Diversity of Arbuscular Mycorrhizal Fungi in the Rhizosphere of Hevea brasiliensis in Hainan Island, China. Phyton (B Aires). 2021;90(1):179–92

56. Zhang S, Luo P, Yang J, Irfan M, Dai J, An N, et al. Responses of Arbuscular Mycorrhizal Fungi Diversity and Community to 41-Year Rotation Fertilization in Brown Soil Region of Northeast China. Front Microbiol. 2021 Oct 11;12. https://doi.org/10.3389/fmicb.2021.742651

57. Zhang M, Shi Z, Yang M, Lu S, Cao L, Wang X. Molecular Diversity and Distribution of Arbuscular Mycorrhizal Fungi at Different Elevations in Mt. Taibai of Qinling Mountain. Front Microbiol. 2021 Mar 4;12. https://doi.org/10.3389/fmicb.2021.609386

58. Cheng Z, Wu S, Du J, Liu Y, Sui X, Yang L. Reduced Arbuscular Mycorrhizal Fungi (AMF) Diversity in Light and Moderate Fire Sites in Taiga Forests, Northeast China. Microorganisms. 2023 Jul 19;11(7):1836. https://doi.org/10.3390/microorganisms11071836

Downloads

Published

2024-09-15

How to Cite

Damián-Carrión, D. A., Silva-Padilla, C. F., Cargua-Catagña, F. E., Reyes Reyes, J., & Paredes-Arcos, F. (2024). Evaluation of Arbuscular Mycorrhizal Fungi Associated with Miconia bracteolata Bonpl case study High Andean Forest, Ecuador. BioNatura Journal: Ibero-American Journal of Biotechnology and Life Sciences, 1(3), 19. https://doi.org/10.70099/BJ/2024.01.03.10

Issue

Section

Research Articles

Categories