From Hack and Tip to AI Precision: the Evolution of CRISPR gRNA Design Invited

Authors

DOI:

https://doi.org/10.70099/BJ/2025.02.03.15

Keywords:

CRISPR, gRNA Design, Genome Editing, Artificial Intelligence, Deep Learning, Machine Learning, Computational Biology, Bioinformatics, CRISPR-Cas9

Abstract

The CRISPR/Cas9 technology is a game-changer in the field of molecular genetics, enabling the first rapid and efficient genomic editing in virtually any organism. The gRNA is a crucial factor in the technology that determines editing efficiency and specificity. The field has progressively moved away from empirical recipes towards computer-aided precision-engineering methodologies based on artificial intelligence (AI) and deep learning over the last decade. This review discusses important breakthroughs in gRNA design, remaining limitations, and prospects, with an emphasis on translational and biotechnological applications.

References

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Published

2025-09-15

How to Cite

Gonzales-Zubiate, F. A. (2025). From Hack and Tip to AI Precision: the Evolution of CRISPR gRNA Design Invited. BioNatura Journal: Ibero-American Journal of Biotechnology and Life Sciences, 2(3), 4. https://doi.org/10.70099/BJ/2025.02.03.15

Issue

Vol. 2 No. 3 (2025)

Section

Perspectives

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