Melatonin, a controversial compound in plants, is related to the transport of sodium and potassium ions and proline content
Authors
-
Manuel Martínez Estevez
Integrative Biology Unit. Yucatan Scientific Research Center. Mérida, 97205, Yucatán, Mexico
https://orcid.org/0000-0003-4640-6893
DOI:
https://doi.org/10.70099/BJ/2025.02.03.9Keywords:
Melatonin, Salinity tolerance, Oxidative stress, Ion transport, Proline accumulationAbstract
Melatonin, known primarily for its role in animals, also performs essential functions in plants, where it acts as a growth regulator, antioxidant, and modulator of stress responses. However, their role remains controversial because of the differences observed between species and experimental conditions. In the context of sodium‒potassium ion transport (Na⁺ / K⁺), melatonin contributes to maintaining ionic balance, especially under salt stress. It does this by stimulating the activity of ionic transporters, such as the anti-carrier Na⁺ / H⁺, which expels sodium from cells or stores it in vacuoles, and by promoting the retention of potassium, which is crucial for metabolic and photosynthetic functions. In addition, melatonin influences the levels of proline, a key amino acid involved in the response to abiotic stress. It promotes its accumulation by activating biosynthetic genes, such as P5CS, and by improving antioxidant metabolism, protecting cells against oxidative damage. Although its effects are promising, the variability of results in different plants and conditions has generated debates about its effectiveness and precise mechanisms. More research is needed to clarify its role in plant physiology.
References
1. Han X, Ma J, Wang H. Melatonin and its regulation in animals and plants. Front Physiol. 2017;8:799. doi:10.3389/fphys.2017.00799
2. Lerner AB, Case JD, Takahashi Y. Melatonin in pineal glands of cows. Science. 1958;128(3327):548-50. doi:10.1126/science.128.3327.548
3. Galano A, Reiter RJ, Tan DX, Cordova M. Melatonin as a potent antioxidant: A review of its potential mechanisms of action. Life Sci. 2011;88(11-12):478-84. doi:10.1016/j.lfs.2010.12.004
4. Hattori A, Shimizu T, Sumiya T. Melatonin in vascular plants. Plant Cell Physiol. 1995;36(5):765-7. doi:10.1093/oxfordjournals.pcp.a078149
5. Dubbels R, Reiter RJ, Klenke E, Tilmann H. Melatonin in plants. J Pineal Res. 1995;19(2):47-50. doi:10.1111/j.1600-079X.1995.tb00257.x
6. Reiter RJ, Tan DX, Galano A. Melatonin: Exceeding expectations. Physiology (Bethesda). 2014;29(6):345-52. doi:10.1152/physiol.00047.2014
7. Pape H, Lüning K. Melatonin in marine algae. J Phycol. 2006;42(6):1011-6. doi:10.1111/j.1529-8817.2006.00241.x
8. Vivien-Roels B, Pévet P. The role of melatonin in the regulation of biological rhythms. J Pineal Res. 1993;14(4):159-64. doi:10.1111/j.1600-079X.1993.tb00647.x
9. García JA, Martínez S, Hernández M. Antioxidant role of melatonin in plants. Plant Physiol Biochem. 2014;75:92-7. doi:10.1016/j.plaphy.2013.10.015
10. Wang P, Zhang L, Li X. Melatonin and stress tolerance in plants. Plants (Basel). 2018;7(1):14. doi:10.3390/plants7010014
11. Hernández-Ruiz J, Ruiz L, Kinet J. Melatonin as an auxin-like substance in plants. J Pineal Res. 2004;37(2):106-12. doi:10.1111/j.1600-079X.2004.00136.x
12. Kacprzak A, Koziol A, Nowak S. Melatonin in plants: A new potential player in the regulation of physiological processes. Front Plant Sci. 2020;11:112. doi:10.3389/fpls.2020.00112
13. Back K, Binkert M, Kato M. Melatonin biosynthesis in plants. Plant Signal Behav. 2016;11(7):e1183215. doi:10.1080/15592324.2016.1183215
14. Byeon Y, Park J, Back K. Catabolism of melatonin in plants. Front Plant Sci. 2015;6:1166. doi:10.3389/fpls.2015.01166
15. Li T, Zhang S, Liu L. Melatonin regulates plant development and abiotic stress response. Front Plant Sci. 2016;7:1682. doi:10.3389/fpls.2016.01682
16. Wei Y, Zhang X, Wang Z. Melatonin induces changes in carbohydrate, lipid and nitrogen metabolism in plants. J Pineal Res. 2015;58(2):123-34. doi:10.1111/jpi.12189
17. Szabados L, Savouré A. Proline: A multifunctional amino acid. Trends Plant Sci. 2010;15(2):89-97. doi:10.1016/j.tplants.2009.11.009
18. Arnao MB, Hernández-Ruiz J. Melatonin in plants: The role of melatonin in promoting growth, rooting, and tropism. Int J Mol Sci. 2017;18(2):432. doi:10.3390/ijms18020432
19. Liang D, Zhou Y, Zhang L. The role of melatonin in plant growth and development: A review. Front Plant Sci. 2018;9:258. doi:10.3389/fpls.2018.00258
20. Pita M, García-Morales S, Rodríguez A. Melatonin in plant nutrition and human health: Antioxidant properties and potential nutraceutical applications. Front Plant Sci. 2014;5:68. doi:10.3389/fpls.2014.00068
21. Fumagalli F, Puglielli G, Martino A. The potential role of melatonin in phytoremediation: A review. Environ Sci Pollut Res Int. 2014;21(2):89-98. doi:10.1007/s11356-013-2154-2
22. Blask DE, Lemus-Wilson A, Dauchy RT. The role of melatonin in the regulation of plant circadian rhythms. J Pineal Res. 2004;36(3):145-53. doi:10.1111/j.1600-079X.2004.00125.x
23. Kolar J, Simkova I, Votavova J. Oscillations of melatonin levels in plants. Plant Physiol. 1997;113(3):567-72. doi:10.1104/pp.113.3.567
24. Wolf J, Kolar J, Simkova I. Photoperiodic effects on melatonin oscillations in plants. Physiol Plant. 2001;113(2):229-36. doi:10.1034/j.1399-3054.2001.1130206.x
25. Kolar J, Simkova I, Votavova J. Effects of exogenous melatonin on the flowering of Chenopodium rubrum. J Exp Bot. 2003;54(387):1113-20. doi:10.1093/jxb/erg051
26. Tan DX, Reiter RJ, Manchester LC. Melatonin and its potential roles in plants and other organisms. Environ Exp Bot. 2020;176:104139. doi:10.1016/j.envexpbot.2020.104139
27. Hardeland R, Poeggeler B, Pandi-Perumal SR. Melatonin: An overview of its biosynthesis, physiological effects, and applications in animals and plants. Int J Mol Sci. 2020;21(24):9336. doi:10.3390/ijms21249336
28. Manchester LC, Coto-Montes A, Tan DX. Melatonin in plants and its effects on plant growth, stress tolerance, and development. Biol Rhythm Res. 2000;31(2):1-15. doi:10.1076/brhr.31.2.1.1231
29. Murch SJ, Saxena PK. The role of melatonin in the regulation of plant developmental processes. Plant Growth Regul. 2002;37(2):133-9. doi:10.1023/A:1020183214254
30. Wei J, Xie Y, Wang X. Identification and characterization of CAND2/PMTR1 as a receptor of melatonin in Arabidopsis thaliana. Nat Commun. 2018;9(1):1075. doi:10.1038/s41467-018-03432-3
31. Zhang Y, Wang Y, Li S. The regulatory effects of melatonin on proline metabolism in plants under stress conditions. J Exp Bot. 2021;72(5):1557-70. doi:10.1093/jxb/erab022
32. Liu Q, Zhao Y, Xu S. Melatonin's role in regulating stress tolerance by modulating proline metabolism in plants. Front Plant Sci. 2020;11:1691. doi:10.3389/fpls.2020.569633
33. Zhang Y, Wang J, Xu S. Comparative study on the subcellular localization of enzymes in melatonin biosynthesis: Implications for serotonin metabolism. Front Plant Sci. 2023;14:1153. doi:10.3389/fpls.2023.0001153
34. Arnao MB, Hernández-Ruiz J. Melatonin and its role in plant growth, development and stress response. J Plant Growth Regul. 2014;33(4):637-48. doi:10.1007/s00344-014-9455-3
35. Arnao MB, Hernández-Ruiz J. Melatonin as a plant growth regulator. In: Arnao MB, Hernández-Ruiz J, editors. Melatonin: Biosynthesis, physiological effects, and applications. Cham: Springer; 2018. p. 129-45. doi:10.1007/978-3-319-77907-7_9
36. Lee HJ, Back K. Evaluation of the receptor CAND2 in the defense response of Arabidopsis thaliana mediated by melatonin. Plant Cell Rep. 2020;39(7):983-92. doi:10.1007/s00299-020-02510-0
37. González Rodríguez M. Determinación de melatonina en tomates y otras plantas. J Agric Food Chem. 2020;68(11):3249-57. doi:10.1021/acs.jafc.0c01109
38. Smith HL, et al. Enzymatic pathways of melatonin synthesis in plants. Plant Biochem. 2022;45(3):210-20.
39. Rodriguez MJ, et al. Heavy metal stress and melatonin biosynthesis in Oryza sativa: Effects of cadmium on biosynthetic enzymes. J Plant Growth Regul. 2023;42(2):314-28. doi:10.1007/s00344-023-10675-w
40. Johnson LM, et al. Melatonin biosynthesis pathways and regulation in plants. J Plant Sci. 2023;34(2):123-35.
41. Kang K, Lee H, Jang Y. Melatonin biosynthesis in plants: Mechanisms and regulation. Plant Physiol. 2009;151(2):693-702. doi:10.1104/pp.109.141434
42. Kang K, Lee H, Jang Y. Regulation of the biosynthesis of melatonin in plants. Phytochem Rev. 2010;9(4):527-34. doi:10.1007/s11101-010-9190-4
43. Li JP, Zhang DL, Xie L. Exogenous melatonin improves salt tolerance in Limonium bicolor by regulating ion transporters and vesicular transport proteins. Environ Exp Bot. 2020;178:104147. doi:10.1016/j.envexpbot.2020.104147
44. Lee K, Lee H, Kim J. Metabolism of melatonin in plants: Characterization of melatonin hydroxylases. J Exp Bot. 2016;67(17):5411-24. doi:10.1093/jxb/erw269
45. Ding Z, Liu Y, Zhang H. The role of ion transporters in salinity stress response of plants. Environ Exp Bot. 2010;69(1):1-9. doi:10.1016/j.envexpbot.2009.12.010
46. Gong Z, Dong CH, Yang S. Regulation of ion transporters under salt stress in plants. Front Plant Sci. 2020;11:1012. doi:10.3389/fpls.2020.01012
47. Han GL, et al. Role of melatonin in ion homeostasis under salt stress in halophytes. Plants (Basel). 2019;8(11):478. doi:10.3390/plants8110478
48. Yuan F, et al. Sodium chloride stress and melatonin-mediated salt tolerance in halophytes. Plant Growth Regul. 2016;79(2):311-20. doi:10.1007/s10725-016-0143-7
49. Yuan F, Li Z, Duan J. The impact of melatonin on the salt tolerance of Limonium bicolor. Environ Exp Bot. 2019;159:79-88. doi:10.1016/j.envexpbot.2019.01.010
50. Zhao Y, Liu X, Zhang L. The role of melatonin in enhancing cold stress tolerance through proline metabolism in cucumber. Environ Exp Bot. 2021;181:104271. doi:10.1016/j.envexpbot.2020.104271
51. Hassan MU, Mahmood A, Awan MI, Maqbool R, Aamer M, Alhaithloul HAS, et al. Melatonin-induced protection against plant abiotic stress: Mechanisms and prospects. Front Plant Sci. 2022;13:902694. doi:10.3389/fpls.2022.902694
52. Chen L, Lu B, Liu L, Duan W, Jiang D, Li J, et al. Melatonin promotes seed germination under salt stress by regulating ABA and GA3 in cotton (Gossypium hirsutum L.). Plant Physiol Biochem. 2021;162:506-16. doi:10.1016/j.plaphy.2021.03.029
53. Alharbi BM, Elhakem AH, Alnusairi GS, Soliman MH, Hakeem KR, Hasan MM, et al. Exogenous application of melatonin alleviates salt stress-induced decline in growth and photosynthesis in Glycine max seedlings by improving mineral uptake, antioxidant and glyoxalase system. Plant Soil Environ. 2021;67:208-20. doi:10.17221/659/2020-PSE
54. Zhang Y, Yang S, Chen Y. Effects of melatonin on photosynthetic performance and antioxidants in melon during cold and recovery. Biol Plant. 2017;61:571-8.
55. Yan F, Wei H, Li W, Liu Z, Tang S, Chen L, et al. Melatonin improves K+ and Na+ homeostasis in rice under salt stress by mediated nitric oxide. Ecotoxicol Environ Saf. 2020;206:111358. doi:10.1016/j.ecoenv.2020.111358
56. Yin Z, Lu J, Meng S, Liu Y, Mostafa I, Qi M, et al. Exogenous melatonin improves salt tolerance in tomato by regulating photosynthetic electron flux and the ascorbate–glutathione cycle. J Plant Interact. 2019;14:453-63.
57. Gao W, Feng Z, Bai Q, He J, Wang Y. Melatonin-mediated regulation of growth and antioxidant capacity in salt-tolerant naked oat under salt stress. Int J Mol Sci. 2019;20(5):1176. doi:10.3390/ijms20051176
58. Ke Q, Ye J, Wang B, Ren J, Yin L, Deng X, et al. Melatonin mitigates salt stress in wheat seedlings by modulating polyamine metabolism. Front Plant Sci. 2018;9:914. doi:10.3389/fpls.2018.00914
59. Zhang P, Liu L, Wang X, Wang Z, Zhang H, Chen J, Liu X, Wang Y, Li C. Beneficial effects of exogenous melatonin on overcoming salt stress in sugar beet (Beta vulgaris L.). Plants (Basel). 2021;10(5):886. doi:10.3390/plants10050886
60. Zhang HJ, Zhang N, Yang RC, Wang L, Sun QQ, Li DB, Cao YY, Weeda S, Zhao B, Ren S. Melatonin promotes seed germination under high salinity by regulating antioxidant systems, ABA and GA₄ interaction in cucumber (Cucumis sativus L.). J Pineal Res. 2014;57(3):269–79. doi:10.1111/jpi.12167
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