Enhanced in vitro propagation of Musa spp. cultivar ‘INIVIT PB-2012’ using VIUSID Agro® as a bio-stimulant
Authors
-
Milagros Basail Pérez
Instituto de Investigaciones de Viandas Tropicales (INIVIT) / Santo Domingo, Villa Clara / Cuba)
https://orcid.org/0000-0003-0486-8600
DOI:
https://doi.org/10.70099/BJ/2025.02.03.7Keywords:
Biostimulant, multiplication coefficient, dose, explantsAbstract
For more than three decades, in vitro techniques have been used in Tissue Culture Biofactories for the propagation of various cultivars. However, the expansion of micropropagation techniques depends on the adaptation of new methodologies that make it possible to increase the multiplication coefficient and improve plant quality. At the Plant Biotechnology Laboratory of the Tropical Root Vegetable Research Institute (INIVIT), with the aim of increasing the multiplication coefficient in the in vitro propagation of the plantain cultivar ‘INIVIT PB-2012’, different concentrations of VIUSID Agro® (0.0 ml.l⁻¹ [control], 0.05 ml.l⁻¹, 0.10 ml.l⁻¹, 0.15 ml.l⁻¹, and 0.20 ml.l⁻¹ of VIUSID Agro®) were tested. The multiplication coefficient (u), height of axillary bud shoots (cm), oxidation degree (u), and pseudostem diameter of axillary bud shoots (cm) were evaluated at 15 days during the in vitro establishment stage and at 21 days during in vitro multiplication after subculture. It was demonstrated that using a dose of 0.15 ml.l⁻¹ of VIUSID Agro® significantly increased the multiplication coefficient from 1.0 (control) to 1.78 in the in vitro establishment stage, with no differences compared to the 0.20 ml.l⁻¹ dose of VIUSID Agro®. In the in vitro multiplication stage, a dose of 0.10 ml.l⁻¹ of VIUSID Agro® produced results not significantly different from those obtained with the 0.15 ml.l⁻¹ dose, increasing the multiplication coefficient from 1.51 (control) to 3.42, as well as improving the other evaluated parameters, resulting in explants of high physiological and genetic quality.
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