Development and Characterization of an Antioxidant Phytocosmetic Gel Based on Thalassia testudinum and Propoline Tincture: Phytochemical, Technological, and Microbiological Evaluation
Authors
- Liz Barbara Pereira Cuni Instituto de Ciencias del Mar (ICIMAR), La Habana, Cuba
-
Idania Rodeiro Guerra
Instituto de Ciencias del Mar (ICIMAR), La Habana, Cuba
https://orcid.org/0000-0002-2692-6050
DOI:
https://doi.org/10.70099/BJ/2025.02.03.4Keywords:
Thalassia testudinum, propoline tincture, phenolic compounds, phytocosmetic antioxidantAbstract
The marine angiosperm Thalassia testudinum, commonly known as turtle grass, is a source for obtaining an extract from the leaves of the species. The extract is rich in phenolic compounds, and within these, the most abundant metabolite is thalassiolin B, a glycosylated flavone with bioactive properties. The present work proposes the development of a gel combining T. testudinum extract and propoline tincture. For this purpose, the main quality control parameters of T. testudinum extract and propoline tincture were determined. In the study, the concentrations of polyphenols, flavonoids, and the antioxidant activity were determined by the DPPH method. Also, the designed formulation was evaluated for physicochemical and technological parameters, and microbiological control was performed. In general, the parameters analyzed were in correspondence with the quality specifications previously established for the extract, the tincture, and the formulation. The results justify the feasibility of using the mixture of T. testudinum extract and propoline tincture as an alternative for use in phytocosmetics.
References
1. Roy A, Khan A, Ahmad I, Alghamdi S, Rajab BS, Babalghith AO, Mohammad Y, Alshahrani, Saiful I and Islam M. Flavonoids a bioactive compound from medicinal plants and its therapeutic applications. BioMed Research International, 2022; 2022. https://doi.org/10.1155/2022/5445291.
2. Hernandez BI, Guerra IR, Declerck K, Herrera IJA, Perez NC, Van CG y Vanden BW. Marine seagrass extract of Thalassia testudinum suppresses colorectal tumor growth, motility and angiogenesis by autophagic stress and immunogenic cell death pathways. Marine Drugs, 2021, 19(2), 52.
3. Delgado RL, Gonzalez K, Mesta F, Couder B, Tavarez Z, Zavala R & Vanden BW. Polyphenolic fraction obtained from Thalassia testudinum marine plant and thalassiolin B exert cytotoxic effects in colorectal cancer cells and arrest tumor progression in a xenograft mouse model. Frontiers in Pharmacology, 2020, 11, 592985.
4. García GRU, Cruz SF, Alarcón AFJ, Nieto TA & Gallegos MME. Análisis fitoquímico cualitativo de los extractos acuosos de Thalassia testudinum banks ex köning et sims de la localidad de Champotón, Campeche, México, durante el ciclo anual 2016-2017. Polibotánica, 2019:(48), 151-168.
5. Goodier CS, Barragán NDG, Moscoso FJT, Fernández AJR. Eficacia del propóleo como agente inhibidor de caries. Rev Cubana Investig Bioméd. 2023;42(2).
6. Castañedo HZA, Meneses MAI, Águila JE, Marrero CO y Seijo WM. Estudios de seguridad toxicológica de la Tintura Hidroalcohólica de Propóleos producida en el Centro de Bioactivos Químicos 2022.
7. Miranda MM, Cuéllar AC. Manual de prácticas de laboratorio. Farmacognosia y productos naturales. La Habana 2000; 25-49,74-79.
8. Rashid MO, Akter M, Uddin J, Islam S, Rahman M, Jahan K, Rahman M, Sadik G. Antioxidant, cytotoxic, antibacterial and thrombolytic activities of Centella asiatica L.: possible role of phenolics and flavonoids. Clinical Phytoscience 2023; 9: 1-9. DOI: 10.1186/s40816-023-00353-8
9. Tabart J, Kevers C, Pincemail J, Defraigne JO and Dommes J. Comparative antioxidant capacities of phenolic compounds measured by various tests. Food chemistry 2009; 113(4), 1226-1233.
10. Iraizoz A, Bilbao O y Barrios MA. Conferencias de tecnología farmacéutica II. La Habana: Enpes 1990; 124.
11. Regulación No. 28. Requerimientos de los estudios de estabilidad para el registro de productos farmacéuticos nuevos y conocidos. República de cuba. Ministerio de Salud Pública. Centro para el Control Estatal de la Calidad de los Medicamentos (CECMED) 2000; 4-5.
12. PNO-LAB 12-002. Edición 02-02. Determinación de caracteres organolépticos, pH y extensibilidad de geles, cremas y ungüentos. La Habana: Empresa Laboratorio Roberto Escudero Díaz 2002.
13. NC 68:2015 Cosméticos- Limites microbianos- Determinaciones.
14. Cedeño FA, Garofalo CB & García MS. Caracterización del propóleo del cantón Rocafuerte y su aplicación en la elaboración de un producto cosmético. MQRInvestigar, 2022: 6(4), 438-454.
15. Shin HJ, Choi SW & Ok G. Qualitative identification of food materials by complex refractive index mapping in the terahertz range. Food Chemistry 2018; 245:282-288. http://dx.doi.org/10.1016/j.foodchem.2017.10.056
16. Alba de Armas, M. Á., Saucedo Hernández, Y., Sotolongo Moya, L., Norman Montenegro, O., Gómez Saucedo, M. L., & Jorge Rodríguez, M. E. (2022). Optimización de la tecnología de la elaboración de la tintura de propolina. Centro Azúcar, 49(3), 69-77.
17. Vasquez FCX e Isuiza Tamayo ARD. Técnicas de remoción de fenoles en aguas residuales industriales 2022
18. Echegaray N, Pateiro M, Munekata PES, Lorenzo JM, Chabani Z, Farag MA and Domínguez, R. Measurement of antioxidant capacity of meat and meat products: Methods and applications. Molecules 2021; 26, 3880.
19. Shraim AM, Ahmed TA, Rahman M, Hijji YM. Determination of total flavonoid content by aluminum chloride assay: A critical evaluation. LWT - Food Science and Technology 2023; 150. Disponible en: https://doi.org/10.1016/j.lwt.2021.111932
20. Febrika ZN, Fujiko M, Dachi K, Nerdy N, Wira SA. Analysis of flavonoid content and antioxidant activity of Solanum ertianthum Extract. IOP Conf. Series: Earth and Environmental Science 2024; 1356. DOI: 10.1088/1755-1315/1356/1/012103
21. Mousavi SS, Karami A, Haghighi TM, Alizadeh S & Maggi F. Phytotoxic potential and phenolic profile of extracts from Scrophularia striata. Plants, 2021: 10(1), 135.
22. Yuan Y, Tang X, Jia Z, Li C, Ma J, Zhang J. The effects of ecological factors on the main medicinal components of Dendrobium offcinale under different cultivation modes. Forests 2020; 11(94). https://doi.org/10.3390/f11010094.
23. Ochoa NA, Rangel R, Ynalvez MA & Ynalvez RA. Antioxidant activity and phytochemical screening in Acacia rigidula Benth. leaves. Advances in Bioscience and Biotechnology, 2024:15(1), 15-38.
24. Sharma R, & Kaul V. Estimation of antioxidant activity and quantification of specific phenols and flavonoids in Asphodelus tenuifolius Cavan.(Onion weed) using HPLC. Vegetos, 2025:38(2), 632-641.
25. Kumar P, Das A. Acid mantle: What we need to know. Indian J. Dermatol. Venereol. Leprol. 2023; 89:729-732.
26. Choi EH, Kang H. Importance of stratum corneum acidification to restore skin barrier function in eczematous diseases. Ann. Dermatol. 2024; 36:1-8.
27. Mijaljica D, Townley J.P, Klionsky D.J, Spada F, Lai M. The Origin, Intricate Nature, and Role of the Skin Surface pH (pHSS) in Barrier Integrity, Eczema, and Psoriasis. Cosmetics 2025; 12, 24. Disponible en: https://doi.org/10.3390/cosmetics12010024
28. Sharma U, Arjariya S, Chouksey R, Sharma N. A Review: Formulation and Evaluation of Pharmaceutical Gel. Journal of Pharmaceutical Negative Results 2022; 13(1):1344-1362. Disponible en: https://doi.org/10.47750/pnr.2022.13.S01.160
29. Metta S, Mohammed AK, Sai LM, Devi P, Shruthi K. A Review: Pharmaceutical Gels and Its Types with Prominence Role of Its Drug Delivery Systems. International Journal of Research and Analytical Reviews 2023; 10(2):686-701. Disponible en: at: https://www.researchgate.net/publication/372195691
30. Bandyopadhyay R, Selvakumar K, Mohamed JMM, Ebrahim D. A Review of Process Validation of Hydrogel Formulation. Int J Pharm Phytopharmacol Res. 2024; 14(1):36-42. Disponible en: https://doi.org/10.51847/fXduKIeSez
Published
How to Cite
Issue
Section
Categories
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with BioNatura Journal agree to the following terms: Authors retain copyright and grant the BioNatura Institutional Publishing Consortium (BIPC) right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0). This allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
