Chromatographic and Microbiological Characterization of Soil Samples from Plots in the Central Commune of Alangasí Village

Authors

DOI:

https://doi.org/10.70099/BJ/2025.02.01.17

Keywords:

soil, forest fire, bacteria, organic matter, chromatography, phosphorus, glucose, cangahua, soil quality, microbial diversity

Abstract

Soil is a vital ecosystem for the survival of all species on the planet. This resource is at risk due to erosive processes caused by natural factors and intensified by anthropogenic activities. This study evaluated land quality affected and unaffected by forest fires in the Central Commune of Alangasí Village. Five plots (197, 200, 205, 274, and 274B) were assessed through qualitative (Pfeiffer chromatography) and quantitative (LOI gravimetric method) tests. Preliminary characterization of bacteria from these areas and complementary measurements of phosphorus and glucose levels were conducted. The results showed that plot 205 (unaffected) had the best soil quality, while plot 197 (affected by fire) exhibited slight recovery. In contrast, plot 200 (cangahua-type soil) presented the poorest condition. Additionally, a predominance of Gram-positive bacteria was found, especially in plots affected by fire (197) and those with higher cangahua content (200). Total phosphorus accumulation and a strong correlation between glucose levels and bacterial growth were observed in the burned plot. The study concluded that vegetation cover, nutrient content, and physicochemical conditions significantly influence soil health.

References

1. Hernández, H.; Sato, M. ¿ La Palma Aceitera (Elaeis Guineensis) Genera Un Impacto Negativo Sobre El Suelo? Agronomía Mesoamericana 2023, 34 (1).

2. Nayakekorale, H. B. Soil Degradation. The Soils of Sri Lanka 2020, 9, 103–118. https://doi.org/10.1007/978-3-030-44144-9_9.

3. Borrelli, P.; Robinson, D. A.; Panagos, P.; Lugato, E.; Yang, J. E.; Alewell, C.; Wuepper, D.; Montanarella, L.; Ballabio, C. Land Use and Climate Change Impacts on Global Soil Erosion by Water (2015-2070). Proceedings of the National Academy of Sciences 2020, 117 (36), 21994–22001. https://doi.org/10.1073/pnas.2001403117.

4. Zhao, J.; Wang, Z.; Dong, Y.; Yang, Z.; Govers, G. How Soil Erosion and Runoff Are Related to Land Use, Topography and Annual Precipitation: Insights from a Meta-Analysis of Erosion Plots in China. Science of The Total Environment 2022, 802, 149665. https://doi.org/10.1016/j.scitotenv.2021.149665.

5. Fontúrbel, T.; Jiménez, E.; MerinoA.; Vega, J. A. Contrasting Immediate Impact of Prescribed Fires and Experimental Summer Fires on Soil Organic Matter Quality and Microbial Properties in the Forest Floor and Mineral Soil in Mediterranean Black Pine Forest. Science of The Total Environment 2023, 167669–167669. https://doi.org/10.1016/j.scitotenv.2023.167669.

6. Molina Velásquez, E. R.; Sinchi Alba, D. I.; Baquero Padilla, P. A. Evaluación de Los Factores Geográficos Que Inciden En Los Flujos Turísticos de La Parroquia Rural de Alangasí. REVISTA CIENTÍFICA ECOCIENCIA 2020, 7 (5), 31–56. https://doi.org/10.21855/ecociencia.75.400.

7. Konrad Miotliński; Kuenzang Tshering; Boyce, M. C.; Blake, D.; Horwitz, P. Simulated Temperatures of Forest Fires Affect Water Solubility in Soil and Litter. Ecological Indicators 2023, 150, 110236–110236. https://doi.org/10.1016/j.ecolind.2023.110236.

8. Vidal-Riveros, C.; Souza-Alonso, P.; Bravo, S.; Laino, R.; Ngo Bieng, M. A. A Review of Wildfires Effects across the Gran Chaco Region. Forest Ecology and Management 2023, 549, 121432. https://doi.org/10.1016/j.foreco.2023.121432.

9. Cruz, H.; Jácome, S.; Gualotuña, T.; Marcillo, D.; Fonseca, R. Causes and Variables of Forest Fires, a Brief Review of the Ecuadorian Case. Springer eBooks 2022, 397–410. https://doi.org/10.1007/978-3-030-96043-8_31.

10. Ministerio del Ambiente. Gobierno Nacional presentó campaña de prevención de incendios forestales – Ministerio del Ambiente, Agua y Transición Ecológica. www.ambiente.gob.ec. https://www.ambiente.gob.ec/gobierno-nacional-presento-campana-de-prevencion-de-incendios-forestales/.

11. Li, Q.; Wang, L.; Fu, Y.; Lin, D.; Hou, M.; Li, X.; Hu, D.; Wang, Z. Transformation of Soil Organic Matter Subjected to Environmental Disturbance and Preservation of Organic Matter Bound to Soil Minerals: A Review. Journal of Soils and Sediments 2022. https://doi.org/10.1007/s11368-022-03381-y.

12. Keiji Jindo; Audette, Y.; Fabio Lopez Olivares; Luciano Pasqualoto Canellas; D. Scott Smith; R. Paul Voroney. Biotic and Abiotic Effects of Soil Organic Matter on the Phytoavailable Phosphorus in Soils: A Review. Chem. Biol. Technol. Agric 2023, 10 (1). https://doi.org/10.1186/s40538-023-00401-y.

13. Urbanski, L.; Karsten Kalbitz; Rethemeyer, J.; Schad, P.; Kögel-Knabner, I. Unexpected High Alkyl Carbon Contents in Organic Matter-Rich Sandy Agricultural Soils of Northwest Central Europe. Geoderma 2023, 439, 116695–116695. https://doi.org/10.1016/j.geoderma.2023.116695.

14. Błońska, E.; Wojciech Prażuch; Lasota, J. Deadwood Affects the Soil Organic Matter Fractions and Enzyme Activity of Soils in Altitude Gradient of Temperate Forests. Forest Ecosystems, 2023, 10, 100115–100115. https://doi.org/10.1016/j.fecs.2023.100115.

15. Mumzaei, A.; Sadeghi, S. H.; Zarei Darki, B.; Homaee, M. Reducing Gasoil-Induced Soil Loss Using Endemic Soil Microorganisms. Physics and Chemistry of the Earth, Parts A/B/C 2024, 135, 103648. https://doi.org/10.1016/j.pce.2024.103648.

16. Meyer, C.; Jeanbille, M.; Breuil, M.-C.; Bru, D.; Höfer, K.; Screpanti, C.; Philippot, L. Soil Microbial Community Fragmentation Reveals Indirect Effects of Fungicide Exposure Mediated by Biotic Interactions between Microorganisms. Journal of Hazardous Materials 2024, 134231. https://doi.org/10.1016/j.jhazmat.2024.134231.

17. Vijay Kumar Aralappanavar; Mukhopadhyay, R.; Yu, Y.; Liu, J.; Bhatnagar, A.; Sarva Mangala Praveena; Li, Y.; Paller, M.; Adyel, T. M.; Jörg Rinklebe; Bolan, N. S.; Sarkar, B. Effects of Microplastics on Soil Microorganisms and Microbial Functions in Nutrients and Carbon Cycling – a Review. Science of the total environment 2024, 924, 171435–171435. https://doi.org/10.1016/j.scitotenv.2024.171435.

18. Liu, Z.; Wu, Z.; Tian, F.; Liu, X.; Li, T.; He, Y.; Li, B.; Zhang, Z.; Yu, B. Phosphate-Solubilizing Microorganisms Regulate the Release and Transformation of Phosphorus in Biochar-Based Slow-Release Fertilizer. Science of The Total Environment 2023, 869, 161622–161622. https://doi.org/10.1016/j.scitotenv.2023.161622.

19. Liang, X.; Zhu, Y.; Liu, H.; Xie, Z.; Li, G.; Li, D.; Liang, Y.; Peng, C. Nitrogen-Fixing Cyanobacteria Enhance Microbial Carbon Utilization by Modulating the Microbial Community Composition in Paddy Soils of the Mollisols Region. Science of the total environment 2024, 172609–172609. https://doi.org/10.1016/j.scitotenv.2024.172609.

20. Suarez, A.; Gwozdz, W. On the Relation between Monocultures and Ecosystem Services in the Global South: A Review. Biological Conservation 2023, 278, 109870. https://doi.org/10.1016/j.biocon.2022.109870.

21. Aliyat, F. Z.; Maldani, M.; Mohammed , E. G.; Nassiri, L.; Ibijbijen, J. Isolation and Characterization of Phosphate Solubilizing Bacteria from Phosphate Solid Sludge of the Moroccan Phosphate Mines. The Open Agriculture Journal 2020, 14 (1), 16–24. https://doi.org/10.2174/1874331502014010016.

22. Mendoza, R.; Espinoza, A. Guía Técnica Para Muestreo de Suelos ASA AGUA Y SUELO PARA LA AGRICULTURA; 2017. https://repositorio.una.edu.ni/3613/1/P33M539.pdf.

23. Red de Buenas Prácticas Agropecuarias. Recomendaciones Para Muestreo de Suelos; 2020. https://redbpa.org.ar/wp-content/uploads/2021/04/EP-RedBPA-MuestreoDe-Suelos.pdf.

24. Infostat. Infostat - Statistical software. www.infostat.com.ar. https://www.infostat.com.ar/.

25. Restrepo, J., & Pinheiro, S. (2011). Cromatografía imagen de vida y destrucción del suelo (1ra ed). Feriva S.A.

26. Ma, Z.; Gali-Izard, T. BeingAliveLanguage: Visualizing Soil Information from a Design Perspective to Enhance Multidisciplinary Communication. Ecological Informatics 2023, 76, 102151–102151. https://doi.org/10.1016/j.ecoinf.2023.102151.

27. Shi, J.; Deng, L.; Yang, L.; Dong, Y.; Liao, Y.; Li, J.; Liu, Y.; Ren, C.; Yang, F.; Zhouping Shangguan; Yakov Kuzyakov. Deciphering Microbial Drivers of Soil Organic Matter Mineralization in Surface and Subsurface Soil during Long-Term Vegetation Succession. Agriculture, ecosystems & environment 2024, 374, 109186–109186. https://doi.org/10.1016/j.agee.2024.109186.

28. Domingues, S.; Boff, P.; Carissimi Boff, M. I. Cromatografía Circular Pfeiffer En Suelo Tratado Con Altas Diluciones Dinamizadas. Revista Mexicana de Ciencias Agrícolas 2022, 13 (7), 1183–1194. https://doi.org/10.29312/remexca.v13i7.3172.

29. Oliveira, W. S.; Costa, J. L. do V.; Cardoso, E. P.; Teixeira, R. de N. P.; Oliveira, M. E. da S. Métodos de Interpretação Para Teste de Qualidade Em Solos a Partir Da Cromatografia Circular Plana (FCC) / Interpretation Methods for Soil Quality Testing from Flat Circular Chromatography (FCC). Brazilian Journal of Animal and Environmental Research 2020, 3 (3), 1107–1125. https://doi.org/10.34188/bjaerv3n3-031.

30. Hu, M.; Wang, J.; Lu, L.; Gui, H.; Wan, S. Global Recovery Patterns of Soil Microbes after Fire. Soil Biology and Biochemistry 2023, 183, 109057. https://doi.org/10.1016/j.soilbio.2023.109057.

31. Mittal, D.; Shukla, R.; Verma, S.; Sagar, A.; Verma, K. S.; Pandey, A.; Negi, Y. S.; Saini, R. V.; Saini, A. K. Fire in Pine Grown Regions of Himalayas Depletes Cultivable Plant Growth Promoting Beneficial Microbes in the Soil. Applied Soil Ecology 2019, 139, 117–124. https://doi.org/10.1016/j.apsoil.2019.03.020.

32. Lucas-Borja, M. E.; Miralles, I.; Ortega, R.; Plaza-Álvarez, P. A.; González-Romero, J.; Sagra, J.; Soriano-Rodríguez, M.; Certini, G.; Heras, J. Immediate Fire-Induced Changes in Soil Microbial Community Composition in an Outdoor Experimental Controlled System. Science of The Total Environment 2019, 696, 134033–134033. https://doi.org/10.1016/j.scitotenv.2019.134033.

33. Cheng, Z.; Wu, S.; Du, J.; Pan, H.; Lu, X.; Liu, Y.; Yang, L. Variations in the Diversity and Biomass of Soil Bacteria and Fungi under Different Fire Disturbances in the Taiga Forests of Northeastern China. Forests 2023, 14 (10), 2063–2063. https://doi.org/10.3390/f14102063.

34. Nwokeh, U. J.; Nwachukwu, S. C.; Onwuka, M. I. EFFECT of TIMING SLASHING, BURNING and SOIL AMENDMENT on SOIL MICROBES. Fudma Journal of Sciences 2023, 6 (5), 161–168. https://doi.org/10.33003/fjs-2022-0605-1186.

35. Lombao, A.; Barreiro, A.; Fonturbel, T.; A. Martín; T. Carballas; Montserrat Díaz-Raviña. Effect of Repeated Soil Heating at Different Temperatures on Microbial Activity in Two Burned Soils. Science of The Total Environment 2021, 799, 149440–149440. https://doi.org/10.1016/j.scitotenv.2021.149440.

36. Pressler, Y.; Moore, J. C.; Cotrufo, M. F. Belowground Community Responses to Fire: Meta-Analysis Reveals Contrasting Responses of Soil Microorganisms and Mesofauna. Oikos 2018, 128 (3), 309–327. https://doi.org/10.1111/oik.05738.

37. Barba, P.; Chimbo, G.; Chiliquinga, A.; Echeverria, C.; Ortega, S.; Sandoval, C.; Zárate, S. Uso de microorganismos nativos como estrategia de restauración de suelos de páramo afectados por incendios. Catálisis. https://www.catalisisec.com/v4-n8-restauracion-paramos.

38. Carrión Granja, B. D. Evaluación Del Efecto de Microorganismos En Las Propiedades Físicas Y Químicas de Suelos Cangahua. Trabajo De titulación, Universidad de las Fuerzas Armadas ESPE, 2020. https://repositoriobe.espe.edu.ec/server/api/core/bitstreams/a416992b-561c-44f1-8f5c-951e2c5bd2bc/content.

39. Díaz, V. C.; Khalajabadi, S. S. ADSORCIÓN de FÓSFORO EN SUELOS de LA ZONA CAFETERA de COLOMBIA. Cenicafé 2018, 69 (2), 7–16.

40. Wu, W.; Wang, F.; Xia, A.; Zhang, Z.; Wang, Z.; Wang, K.; Dong, J.; Li, T.; Wu, Y.; Che, R.; Li, L.; Niu, S.; Hao, Y.; Wang, Y.; Cui, X. Meta-Analysis of the Impacts of Phosphorus Addition on Soil Microbes. Agriculture, Ecosystems & Environment 2022, 340, 108180. https://doi.org/10.1016/j.agee.2022.108180.

41. Zhou, Z.; Wang, C.; Luo, Y. Meta-Analysis of the Impacts of Global Change Factors on Soil Microbial Diversity and Functionality. Nature Communications 2020, 11 (1). https://doi.org/10.1038/s41467-020-16881-7.

42. Chávez, R. S. Respuesta Del Cultivo de Frijol Común a Bajo Contenido de Fósforo En El Suelo: Revisión de Literatura. Trabajo De titulación, Escuela Agrícola Panamericana, Zamorano Honduras, 2020. https://bdigital.zamorano.edu/server/api/core/bitstreams/ac3cf030-d4ac-48a5-bdf2-6d674f9c1bca/content.

43. Zhang, X.; Zhan, Y.; Zhang, H.; Wang, R.; Tao, X.; Zhang, L.; Zuo, Y.; Zhang, L.; Wei, Y.; Li, J. Inoculation of Phosphate-Solubilizing Bacteria (Bacillus) Regulates Microbial Interaction to Improve Phosphorus Fractions Mobilization during Kitchen Waste Composting. Bioresource technology 2021, 340, 125714–125714. https://doi.org/10.1016/j.biortech.2021.125714.

44. Yogender Pal Khasa; Mohanty, S. R. Growth Physiology and Kinetics. Springer eBooks 2021, 137–179. https://doi.org/10.1007/978-981-16-0723-3_5.

45. Schulze, K. L.; Lipe, R. S. Relationship between Substrate Concentration, Growth Rate, and Respiration Rate of Escherichia Coli in Continuous Culture. Archiv fr Mikrobiologie 1964, 48 (1), 1–20. https://doi.org/10.1007/bf00406595.

Downloads

Published

2025-03-15

How to Cite

Gavilanes, Álvaro P. (2025). Chromatographic and Microbiological Characterization of Soil Samples from Plots in the Central Commune of Alangasí Village. BioNatura Journal: Ibero-American Journal of Biotechnology and Life Sciences, 2(1), 20. https://doi.org/10.70099/BJ/2025.02.01.17

Issue

Section

Research Articles

Categories