Isolation, characterization and identification of klebsiella sp. 35-DOR_27F isolated from textile effluents

Authors

DOI:

https://doi.org/10.70099/BJ/2024.01.01.36

Keywords:

Bacterial strain, Klebsiella sp, DNA extraction, decolorization, effluents

Abstract

This research study aimed to isolate and characterize a new bacterial strain from textile effluents. In order to do this, bacteria were cultured using the MSM medium, where a colony was isolated through six successive pickings. It then underwent a DNA extraction process using the phenol-chloroform-isoamyl alcohol methodology, and an electrophoresis was carried out to confirm the extraction. In addition, the isolated colony was identified as Klebsiella sp. by sequencing the 16S rRNA gene using bioinformatics tools. To observe its biotechnological potential, the bacterial strain was grown in an MSM broth enriched with Golden Yellow K2R azoic dye at a concentration of 50 mg/L, showing a percentage of decolorization of 74 % after 72 hours at 37 °C, indicating the potential of the isolated colony for the development of bio-remediation processes for effluents containing azoic dyes.

References

1. Sunkar S, C. VN, K. R. Citrobacter freundii mediated degradation of textile dye Mordant Black 17. Journal of Water Process Engineering. 2015;8:28-34. doi:10.1016/j.jwpe.2015.08.006

2. Chung KT. The Significance of Azo-Reduction in the Mutagenesis and Carcinogenesis of Azo Dyes. Vol 114.; 1983.

3. Kawakami T, Isama K, Nakashima H, Tsuchiya T, Matsuoka A. Analysis of primary aromatic amines originated from azo dyes in commercial textile products in Japan. J Environ Sci Health A Tox Hazard Subst Environ Eng. 2010;45(10):1281-1295. doi:10.1080/10934529.2010.493827

4. Ito T, Shimada Y, Suto T. Potential use of bacteria collected from human hands for textile dye decolorization. Water Resour Ind. 2018;20:46-53. doi:10.1016/j.wri.2018.09.001

5. Aksu Z, Tezer S. Biosorption of reactive dyes on the green alga Chlorella vulgaris. Process Biochemistry. 2005;40(3-4):1347-1361. doi:10.1016/j.procbio.2004.06.007

6. Hossen MZ, Hussain ME, Hakim A, Islam K, Uddin MN, Azad AK. Biodegradation of reactive textile dye Novacron Super Black G by free cells of newly isolated Alcaligenes faecalis AZ26 and Bacillus spp obtained from textile effluents. Heliyon. 2019;5(7). doi:10.1016/j.heliyon.2019.e02068

7. Valli Nachiyar C, Namasivayam SKR, Kumar RR, Sowjanya M. Bioremediation of textile effluent containing Mordant Black 17 by bacterial consortium CN-1. Journal of Water Process Engineering. 2014;4(C):196-200. doi:10.1016/j.jwpe.2014.10.003

8. Karim ME, Dhar K, Hossain MT. Decolorization of Textile Reactive Dyes by Bacterial Monoculture and Consortium Screened from Textile Dyeing Effluent. Journal of Genetic Engineering and Biotechnology. 2018;16(2):375-380. doi:10.1016/j.jgeb.2018.02.005

9. Garzón-Pérez AS, Paredes-Carrera SP, Martínez-Gutiérrez H, Cayetano-Castro N, Sánchez-Ochoa JC, Pérez-Gutiérrez RM. Effect of combined microwave-ultrasound irradiation in the structure and morphology of hydrotalcite-like compounds al/mg-ch3coo and its evaluation in the sorption of a reactive dye. Revista Mexicana de Ingeniera Quimica. 2020;19(1):363-375. doi:10.24275/rmiq/Mat567

10. Axelsson J, Nilsson U, Terrazas E, Alvarez Aliaga T, Welander U. Decolorize the Reactive Red 2 and Reactive Blue 4 textile dyes using Bjerkandera sp. Strain BOL 13 in a continuous rotating biological contactor reactor. Enzyme Microb Technol. 2006;39(1):32-37. doi:10.1016/j.enzmictec.2005.09.006

11. Kumar SS, Shantkriti S, Muruganandham T, Murugesh E, Rane N, Govindwar SP. Bioinformatics aided microbial approach for bioremediation of wastewater containing textile dyes. Ecol Inform. 2016;31:112-121. doi:10.1016/j.ecoinf.2015.12.001

12. Saratale RG, Saratale GD, Chang JS, Govindwar SP. Bacterial decolorization and degradation of azo dyes: A review. J Taiwan Inst Chem Eng. 2011;42(1):138-157. doi:10.1016/j.jtice.2010.06.006

13. Durai S, Kumar NM, Saravanan D. Isolation of dye degrading bacteria from textile effluent. Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research. 2015;7(3):2214-2218. https://www.researchgate.net/publication/305787431

14. Shah MP. Isolation and Screening of Dye Decolorizing Bacteria. J Appl Environ Microbiol. 2014;2(5):244-248. doi:10.12691/jam-2-5-7

15. Jadhav JP, Kalyani DC, Telke AA, Phugare SS, Govindwar SP. Evaluation of the efficacy of a bacterial consortium for the removal of color, reduction of heavy metals, and toxicity from textile dye effluent. Bioresour Technol. 2010;101(1):165-173. doi:10.1016/j.biortech.2009.08.027

16. Hussain S, Maqbool Z, Ali S, et al. Biodecolorization of reactive black-5 by a metal and salt tolerant bacterial strain Pseudomonas sp. RA20 isolated from Paharang drain effluents in Pakistan. Ecotoxicol Environ Saf. 2013;98:331-338. doi:10.1016/j.ecoenv.2013.09.018

17. Du LN, Li G, Zhao YH, et al. Efficient metabolism of the azo dye methyl orange by Aeromonas sp. strain DH-6: Characteristics and partial mechanism. Int Biodeterior Biodegradation. 2015;105:66-72. doi:10.1016/j.ibiod.2015.08.019

18. Anjaneya O, Souche SY, Santoshkumar M, Karegoudar TB. Decolorization of sulfonated azo dye Metanil Yellow by newly isolated bacterial strains: Bacillus sp. strain AK1 and Lysinibacillus sp. strain AK2. J Hazard Mater. 2011;190(1-3):351-358. doi:10.1016/j.jhazmat.2011.03.044

19. Moosvi S, Kher X, Madamwar D. Isolation, characterization and decolorization of textile dyes by a mixed bacterial consortium JW-2. Dyes and Pigments. 2007;74(3):723-729. doi:10.1016/j.dyepig.2006.05.005

20. Govindwar SP, Kurade MB, Tamboli DP, Kabra AN, Kim PJ, Waghmode TR. Decolorization and degradation of xenobiotic azo dye Reactive Yellow-84A and textile effluent by Galactomyces geotrichum. Chemosphere. 2014;109:234-238. doi:10.1016/j.chemosphere.2014.02.009

21. Enayatizamir N, Tabandeh F, Rodríguez-Couto S, Yakhchali B, Alikhani HA, Mohammadi L. Biodegradation pathway and detoxification of the diazo dye Reactive Black 5 by Phanerochaete chrysosporium. Bioresour Technol. 2011;102(22):10359-10362. doi:10.1016/j.biortech.2011.08.130

22. Chen G, Huang M hong, Chen L, Chen D hui. A batch decolorization and kinetic study of Reactive Black 5 by a bacterial strain Enterobacter sp. GY-1. Int Biodeterior Biodegradation. 2011;65(6):790-796. doi:10.1016/j.ibiod.2011.05.003

23. Brilon C, Beckmann W, Hellwig M, Knackmuss3t HJ. Enrichment and Isolation of Naphthalenesulfonic Acid-Utilizing Pseudomonads.; 1981

24. Of R, Technologists. TECHNICAL SECTION ANTIBIOTIC SUSCEPTIBILITY TESTING BY A

STANDARDIZED SINGLE DISK METHOD. Vol 45.; 1966. https://academic.oup.com/ajcp/article

25. Fujimoto S, Nakagami Y, Kojima F. Optimal Bacterial DNA Isolation Method Using Bead-Beating

Technique. Vol 3.; 2004. https://www.researchgate.net/publication/268409445

26. Zhang Z, Schwartz S, Wagner L, Miller W. A Greedy Algorithm for Aligning DNA Sequences. Vol 7.

Mary Ann Liebert, Inc. Pp; 2000.

27. Ajaz M, Rehman A, Khan Z, Nisar MA, Hussain S. Degradation of azo dyes by Alcaligenes aquatilis 3c and its potential use in the wastewater treatment. AMB Express. 2019;9(1). doi:10.1186/s13568-019-0788-3

28. Mahbub KR, Mohammad A, Ahmed MM, Begum S. Decolorization of Synthetic Dyes Using Bacteria Isolated from Textile Industry Effluent. Asian Journal of Biotechnology. 2012;4(3):129-136. doi:10.3923/ajbkr.2012.129.136

29. Ajaz M, Elahi A, Rehman A. Degradation of azo dye by bacterium, Alishewanella sp. CBL-2 isolated from industrial effluent and its potential use in decontamination of wastewater. Journal of Water Reuse and Desalination. 2018;8(4):507-515. doi:10.2166/wrd.2018.065

Downloads

Published

2024-02-15

How to Cite

Ortiz-Romero , D., Camacho-Valencia, D., & Ramírez-Revilla , S. A. (2024). Isolation, characterization and identification of klebsiella sp. 35-DOR_27F isolated from textile effluents. BioNatura Journal: Ibero-American Journal of Biotechnology and Life Sciences, 1(1), 9. https://doi.org/10.70099/BJ/2024.01.01.36

Issue

Vol. 1 No. 1 (2024)

Section

Research Articles

Categories

License

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with BioNatura Journal agree to the following terms: Authors retain copyright and grant the BioNatura Institutional Publishing Consortium (BIPC) right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0). This allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.